Sample: Ceramide mixture (about 500 ng each)

Column: Betasil DIOL100（4.6mm ID Ｘ150mm L）

Column temp.: 35°C

Mobile phase: A: Chloroform  B: 5％H2O/MeOH

Gradient: 0min→1min→10min、7 ％B→7 ％B→50％B

Flow rate: 1.2ml/min

SofTA ELSD Conditions: Spray Chamber 45°C Drift Tube 55°C Filter 3, Gain Normal

Chromatogram provided by Miyako Kawakatsu and Hiroyuki Kaiho, M&S Instruments