Monitoring of Synthesized Compound with ELSD
The following data shows usefulness of the SofTA ELSD compared with UV detector. As G-OMe does not have UV absorbance, it is difficult to monitor this synthesis reaction with UV detector.
G-OMe(Glycine methylester) + Boc-F (Butoxy phenylalanine) = Boc-F-G-OMe
Column; BETASILC18 4.6 x 150mm L
Col. Temp.; Ambient
Mobile Phase; A_H2O, B_MeOH
Gradient; 0 min 40 % B, 7 min 100 % B, 15 min 100 % B
Flow Rate; 1 ml/min
SofTA conditions;
Blue lineSC temp.; 45 C
DT temp,; 60 C
FLT; 0
UV
； 254 nm (0.001 au 10mV) Red line